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In one mouse study, researchers found that the peptide reduced the pain felt by the animals in their paws when “placed on a mildly hot plate.” The authors explained that this could be because GHK is similar in structure to cimetidine, a histamine H2 receptor antagonist sometimes given to reduce pain in certain digestive conditions.3 10 In rodents, doses as low as 0.5mg/kg were found to be effective in delivering these anti-pain effects.25 In a 70kg human, an equivalent dose would be around 5.6mg, though that’s likely higher than necessary: pharmaceutical-grade GHK-Cu normally ranges from 0.5 mg to 2.5mg per injection

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This setup gives researchers a practical way to study angiogenic upregulation, cytoskeletal dynamics, and extracellular matrix (ECM) remodelling all at once, rather than running separate isolated assays