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Diffusion Smart-seq3D reveals genes and pathways with diverse spatial expression pattern Examining the linear and quadratic parameters of the spatial expression functions inferred for the different genes of our dataset classifies genes into four patterns: genes expressed mainly in the spheroid core, genes expressed mainly in the periphery, genes expressed in the intermediate region half-way in between the core and the periphery, and genes expressed at extrema of the core-periphery axis but not in in the intermediate region (Fig